RhD Specific Antibodies Are Not Detectable in HLA-DRB11501*Mice Challenged with Human RhD Positive Erythrocytes
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چکیده
منابع مشابه
RhD Specific Antibodies Are Not Detectable in HLA-DRB1*1501 Mice Challenged with Human RhD Positive Erythrocytes
The ability to study the immune response to the RhD antigen in the prevention of hemolytic disease of the fetus and newborn has been hampered by the lack of a mouse model of RhD immunization. However, the ability of transgenic mice expressing human HLA DRB1(*)1501 to respond to immunization with purified RhD has allowed this question to be revisited. In this work we aimed at inducing anti-RhD a...
متن کاملMolecular cloning of RhD cDNA derived from a gene present in RhD-positive, but not RhD-negative individuals.
The Rh blood group system plays a major role in immune and nonimmune hemolytic states. Although an Rh cDNA has been previously cloned, there is no information on which Rh antigenic protein it encodes. Using polymerase chain reaction (PCR) amplification, we have identified this original Rh clone, here designated Rh21, and an additional Rh cDNA clone, Rh13, that is 96% nucleotide- and 92% amino a...
متن کاملThe RHD gene is highly detectable in RhD-negative Japanese donors.
Recent molecular studies on the Rh blood group system have shown that the Rh locus of each haploid RhD-positive chromosome is composed of two structural genes: RHD and RHCE, whereas the locus is made of a single gene (RHCE) on each haploid RhD-negative chromosome. We analyzed the presence or absence of the RHD gene in 130 Japanese RhD-negative donors using the PCR method. The RhD-negative pheno...
متن کاملMolecular analysis of Rh polypeptides in a family with RhD-positive and RhD-negative phenotypes.
To investigate the genetic basis of the Rh polypeptide gene, we attempted the isolation of cDNA clones for Rh polypeptide from a family with the RhD-positive and RhD-negative phenotypes using the reverse transcription (RT)-PCR method for each reticulocyte RNAs followed by subcloning. The isolated cDNAs showed the existence of another Rh-related clone (RhPII-1 cDNA, tentative designation) beside...
متن کاملVariant RHD Types in Brazilians With Discrepancies in RhD Typing.
BACKGROUND The knowledge of D variants in patients and donors is important because anti-D alloimmunization can occur in some but not all individuals who express a variant RHD allele. Serologic distinction of RhD discrepancies is not always straightforward, which makes molecular analysis highly desirable. METHODS A group of 223 subjects, 129 patients, and 94 blood donors was identified and ana...
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ژورنال
عنوان ژورنال: Advances in Hematology
سال: 2014
ISSN: 1687-9104,1687-9112
DOI: 10.1155/2014/470242